Form: 10KSB

Optional form for annual and transition reports of small business issuers [Section 13 or 15(d), not S-B Item 405]

March 29, 2007


CERTIFICATION OF THE PRINCIPAL EXECUTIVE OFFICER
 
AND PRINCIPAL FINANCIAL OFFICER
 
Pursuant to 18 U.S.C. § 1350, as created by Section 906 of the Sarbanes-Oxley Act of 2002, the undersigned officer of Lixte Biotechnology Holdings, Inc. (the “Company”) hereby certifies that, to his knowledge:
 
(i) The Annual Report on Form 10-KSB of the Company for the fiscal year ended December 31, 2006 (the “Report”) fully complies with the requirements of Section 13(a) or Section 15(d), as applicable, of the Securities Exchange Act of 1934, as amended; and
 
(ii) The information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of the Company.
 
     
Date: March 28, 2007 By:   /s/ John S. Kovach
 
John S. Kovach
  Chief Executive Officer and Chief Financial Officer
 
 
A signed original of this written statement required by Section 906, or other document authenticating, acknowledging, or otherwise adopting the signature that appears in typed form within the electronic version of this written statement required by Section 906, has been provided to the Company and will be retained by the Company and furnished to the Securities and Exchange Commission or its staff upon request.