Published on November 12, 2008
CERTIFICATIONS
        OF THE CHIEF EXECUTIVE OFFICER AND CHIEF FINANCIAL OFFICER 
      UNDER
        SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002
      In
        connection with the filing by Lixte Biotechnology Holdings, Inc. (the
“Registrant”) of its Quarterly Report on Form 10-Q for the quarterly period
        ended September 30, 2008 (the “Quarterly Report”) with the Securities and
        Exchange Commission, I, John S. Kovach, certify, pursuant to 18 U.S.C. Section
        1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002,
        that:
      (1)
        The
        Quarterly Report fully complies with the requirements of Section 13(a) or
        15(d)
        of the Securities Exchange Act of 1934; and
      (2)
        The
        information contained in the Quarterly Report fairly presents, in all material
        respects, the financial condition and results of operations of the
        Registrant.
      A
        signed
        original of this written statement required by Section 906 has been provided
        to
        the Registrant and will be retained by the Registrant and furnished to the
        Securities and Exchange Commission or its staff upon request.
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                 Date:
                  November 12, 2008 
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                 By: 
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                 /s/
                  JOHN S. KOVACH 
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                 John
                  S. Kovach 
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                 Chief
                  Executive Officer and Chief Financial Officer 
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                 (Principal
                  Executive Officer and Principal Financial
                  Officer) 
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