Form: 10KSB/A

Optional form for annual and transition reports of small business issuers [Section 13 or 15(d), not S-B Item 405]

May 17, 2007


CERTIFICATION OF THE PRINCIPAL EXECUTIVE OFFICER
 
AND PRINCIPAL FINANCIAL OFFICER
 
Pursuant to 18 U.S.C. § 1350, as created by Section 906 of the Sarbanes-Oxley Act of 2002, the undersigned officer of Lixte Biotechnology Holdings, Inc. (the “Company”) hereby certifies that, to his knowledge:
 
(i) Amendment No. 2 to this Annual Report on Form 10-KSB of the Company for the fiscal year ended December 31, 2006 (the “Report”) fully complies with the requirements of Section 13(a) or Section 15(d), as applicable, of the Securities Exchange Act of 1934, as amended; and
 
(ii) The information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of the Company.
 
     
Date: May 14, 2007 By:   /s/ John S. Kovach
 
John S. Kovach
  Chief Executive Officer and Chief Financial Officer